LOCUS       40924_23719       12283 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB714 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12283)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12283)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12283)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1424..2080
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2425..2727
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2771..2895)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2924..3013
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      3047..3299
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3333..3349)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3357..3373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3381..3411)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3426..3447)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3483..3735)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3773..5068)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61199.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3773..5068)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5120..5299)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5805..5829)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6350..7138
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7387..7975
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8161..8301)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8645..8839)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8908..9978)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10410..11036)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcgggtt aattaacatc ttttacccat acgatgttcc
     2941 tgactatgcg ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt
     3001 tccagattac gctgctcagt gcagcgctta gagctcgaat ttccccgatc gttcaaacat
     3061 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     3121 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     3181 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     3241 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     3301 ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3421 gcctaatgag tgagctaact cacattaggc tgaattaggc gcgcctattt ctattaattc
     3481 ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     3541 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     3601 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     3661 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     3721 aatgtttgaa cgatcgggga aattcgcctc gagtgacttc tctgattgca gactatttat
     3781 accaaccagc aagaaagtgt cgaagtatga agcagaaggc gcaggctaga gcctggaaaa
     3841 cgagaagatg gatgcaaaga gacttttctg atttaggacc aaacaacctc aagtaaacat
     3901 tgacgagtgt cccatctttt gtgccggtca gtaatccggt agcaggatca taccttggga
     3961 gacgatggcg cggacatttc acaatcccag caagctgagg aagcgataat agaaggttca
     4021 gaacttgagg aagaaagaag atgaggaggg tttcactgaa gtgacccaga ataccaacaa
     4081 ctgccatagt cattccggcg aagactgtgt aagtgtctcc aacaaaaact gaagaaggat
     4141 accaattgta agcaagcatt gccaaggatg tggccatcag cggttgagta agaaatatcg
     4201 agaaggcatg agcttggtga tactcgggat caacagatgc tccgatttgc ataacgttgt
     4261 gtatcaggat agcagcggca ataacaactg tttgaccgat ttcaaggcca ttcagaccag
     4321 cgtggatgtt aatagagttt gtacaaaaga cagcaagtag ccccatatat aacttatata
     4381 tccttcctag attaagcact tccaagccaa tataagcaac aagaggtttt ggtataacaa
     4441 tagttgtatg accagcataa gccatcaaaa gtggaagggt agcaaaagat ggcaggacaa
     4501 gttttactct ccaaggcaca tcaaggacat catctacaaa tccaagcaaa atcatgaagc
     4561 aaatagatgc tagtgctgca ttatactcca caagccaatt agaatcttca gtgaagttaa
     4621 aatactgaaa tattatcgcc acgatcaaaa agacaatacc aacaacaata cccaatgact
     4681 ctggcacttt aatatctcct tgaggcgtgc ctcttttgtt gatatcaaaa ccaaacatgt
     4741 tacgtctcag aacgtatctg gcagtaacag gaatcatttt gagagtgacg aaaaacccaa
     4801 ccaagctaag ccccgcgtta attagaatcg atcgcttgag ttcattatca accttgtagt
     4861 gaaaacagag aaggtataga tagagcgaac agagaagagt agagatcacg aagatcactc
     4921 ctaatttcgg cggcgcgaga cgaaattcct caccggaagc agaaaccgtc tttcgggtca
     4981 tcttctgctc cgatggcgct gagttgggtt ccgacgggtc cggtttgttg ggaattgaga
     5041 tcgatgaagc tctcttacga gctgccatat cttattgccc cccgggatca gatctggatt
     5101 gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc
     5161 atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa
     5221 taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc
     5281 tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg
     5341 cgggggtcat aacgtgactc ccttaattct ccgctcatga tcttgatccc ctgcgccatc
     5401 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
     5461 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
     5521 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
     5581 tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt
     5641 tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag
     5701 ctgatccgtc gagattaata gaaataaatt cagcctaatt cggcgttaat tcagtacatt
     5761 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     5821 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     5881 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     5941 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     6001 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     6061 gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     6121 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     6181 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     6241 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     6301 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
     6361 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     6421 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     6481 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     6541 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     6601 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     6661 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     6721 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     6781 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     6841 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     6901 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     6961 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     7021 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     7081 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     7141 atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     7201 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
     7261 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa
     7321 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7381 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7441 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7501 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     7561 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7621 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7681 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7741 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7801 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7861 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7921 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7981 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8041 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8101 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8161 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     8221 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     8281 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     8341 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     8401 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg
     8461 ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag
     8521 gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag
     8581 ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga
     8641 cagttatgca caggccaggc gggttttaag agttttaata agttttaaag agttttaggc
     8701 ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat
     8761 gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc
     8821 aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat
     8881 ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt
     8941 gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc
     9001 cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc
     9061 ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc
     9121 ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc
     9181 ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc
     9241 agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg
     9301 gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt
     9361 acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt
     9421 ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa
     9481 cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg
     9541 ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc
     9601 ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc
     9661 acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac
     9721 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat
     9781 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc
     9841 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc
     9901 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga
     9961 tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc
    10021 cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat
    10081 tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa
    10141 attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga
    10201 tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc
    10261 ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa
    10321 cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt
    10381 gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc
    10441 gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg
    10501 gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg
    10561 tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac
    10621 acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc
    10681 accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag
    10741 tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact
    10801 acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg
    10861 acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga
    10921 tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt
    10981 gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt
    11041 tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca
    11101 agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg
    11161 ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca
    11221 gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg
    11281 cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc
    11341 ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc
    11401 ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag
    11461 tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg
    11521 gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg
    11581 ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat
    11641 gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc
    11701 cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg
    11761 gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt
    11821 cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc
    11881 gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc
    11941 ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc
    12001 agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt
    12061 tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg
    12121 cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa
    12181 gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc
    12241 ctgtggttgg catgcacata caaatggacg aacggataaa cct
//
